For Mitochondria and Cell Suspensions
The base unit houses the fixed-speed solid-state magnetic stirrer. The rear of the unit has attachments for connecting the unit to a pumped source of constant temperature water. The SI130 microcathode oxygen electrode, which forms the floor of the respiration chamber, is inserted from below.
The chamber unit is sealed against the base unit by a threaded acetal collar. By unscrewing this the chamber unit can be removed for cleaning. The glass chamber unit comprises an outer water jacket through which constant temperature water is passed, and a central precision bore tube. The chamber is formed by the insertion of the polycarbonate plunger. This can be inserted to one of two (or three for the MT200A) different depths depending on the volume of respiration chamber required. Calibration of chamber volume is by means of a gauge piece, which is supplied.
Substrate or inhibitor can be added during the course of a respirometry run via the capillary bore in the plunger. A 1µl (MT200 glass) or 5µl (MT200A glass) syringe it supplied for this. Stainless steel spinbars are provided in order to stir the contents of the chamber.