If you wish to set up a Respirometry System, you will require to purchase:
- 782 meter
- 1302 electrode (1 or 2)
- Electrode service kit
- Respirometer cell (MT200, MT200A, RC300, RC350, RC400)
In addition, you will need to provide a pumped source of constant temperature water and a PC.
For Respirometry, O2 Monitoring and Spot O2 Measurements
- Operates with 1 or 2 microcathode oxygen electrodes
- Selection of oxygen measuring units
- Closed cell or flow cell respirometry or monitoring applications
- Supplied with sophisticated, easy-to-use software
- Automatic calculation and reporting of respiration rates
- Facility for off-line storage of oxygen readings if required.
The model 782 is a new concept in precision dissolved oxygen measurement. It acts as either a stand-alone oxygen meter or it can be connected to the Strathkelvin respirometry/monitoring software (supplied) on your PC. Either one or two 1302 electrodes can be connected to the meter. The digital display can be set to read in units of PO2 (torr, mm Hg, kPa) or concentration (% saturation, ppm, mg/l, or µmol/l).
The software is virtually identical to the 929 system software. The only difference is that you will see only 1 or 2 traces displayed instead of 6. A comprehensive, easy to follow, instruction manual is supplied, which will be particularly valuable to anyone who is not familiar with respirometry work.
If you would like to see simulations of the software in action please click here to download demo or go to contact us to request a demonstration disk cd.
For Mitochondria and Cell Suspensions
The base unit houses the fixed-speed solid-state magnetic stirrer. The rear of the unit has attachments for connecting the unit to a pumped source of constant temperature water. The SI130 microcathode oxygen electrode, which forms the floor of the respiration chamber, is inserted from below.
The chamber unit is sealed against the base unit by a threaded acetal collar. By unscrewing this the chamber unit can be removed for cleaning. The glass chamber unit comprises an outer water jacket through which constant temperature water is passed, and a central precision bore tube. The chamber is formed by the insertion of the polycarbonate plunger. This can be inserted to one of two (or three for the MT200A) different depths depending on the volume of respiration chamber required. Calibration of chamber volume is by means of a gauge piece, which is supplied.
Substrate or inhibitor can be added during the course of a respirometry run via the capillary bore in the plunger. A 1µl (MT200 glass) or 5µl (MT200A glass) syringe it supplied for this. Stainless steel spinbars are provided in order to stir the contents of the chamber.
