Multi-Cell Respirometer
RC650
Six-cell respirometer for small volume replicate respiration measurements of oxygen uptake by microbial and cells suspensions, mitochondria or enzyme preparations.
Features
- Ability to carry out replicate experiments
- Able to utilise the advanced recording and analysis features of the SI929 Six-Channel Oxygen Meter.
- Integral unit, complete with magnetic stirrer
- Good visibility of cell contents
- Variable cell volume of 1-3 ml
Overview
The RC650 Multi-Cell Respirometer has been designed by Strathkelvin Instruments to enable up to six simultaneous closed-cell respiration measurements to be made in volumes of 1-3 ml.
The Multi-Cell Respirometer is designed to be used in conjunction with the Strathkelvin Instrument's SI929 Six-Channel Oxygen Meter.
The Multi-Cell Respirometer comprises a sealed water bath containing six respirometer cells located above a six-position magnetic stirrer. A black acetal electrode holder, with a 5 mm hole in its tip through which the membrane of the SI1302 Electrode protrudes, makes a sliding fit into the precision bore cell. As the holder is inserted into the preparation, the sloping face at its tip gathers the air into the slot which runs the complete length of the holder.
Respiratory inhibitors or other solutions can be introduced directly into the cell via this slot, using a fine bore needle with luer fitting, which is supplied. The volume of the cell may be set between 1 and 3 ml, by rotating the knurled collar of the holder.
The water bath is fitted with 8 mm stainless steel water inlets to allow connection to a pumped source of constant temperature water. The rear of the bath contains a further six cells which are used to park the electrode holders and maintain them at constant temperature while changing the contents of the respirometer cells. All cells are sealed into the water bath with O-rings, so that they can be removed, if necessary, for cleaning.
For fast reactions, like those removing all of the oxygen in 20 minutes or less, it is recommended that the electrodes are fitted with FEP membranes which require a special electrode jacket.